Read e-book online Current Topics in Membranes, Volume 63 PDF

By Larry DeLucas

ISBN-10: 0123749875

ISBN-13: 9780123749871

This quantity of present issues in Membranes makes a speciality of Membrane Protein Crystallization, starting with a overview of previous successes and common developments, then extra discussing demanding situations of mebranes protein crystallization, mobile unfastened construction of membrane proteins and novel lipids for membrane protein crystallization. This ebook additionally comprises instruments to enchance membrane protein crystallization, procedure developments, and crystallization options used for photosystem I and its complexes, setting up Membrane Protein Crystallization as a wanted, sensible reference for researchers.

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Extra resources for Current Topics in Membranes, Volume 63

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Selected E‐PCR products were used directly without the need for further purification as templates for linked transcription‐translation reactions in the insect cell‐free system. Radio‐labeled in vitro translation products were separated on an SDS gel 40 Kubick et al. 0- Hb-EGF- TRPV1- TRPV1- ETB-GFP AQP 6 UCP1 UCP4 OGCP YFP CFP YFP TRP V4 FIGURE 7 Productivity of the batch‐formatted insect cell lysates. In vitro translation reactions were supplemented with mRNA encoding individual membrane proteins.

Luciferase Activity Assay Luciferase activity was determined using the Luciferase Assay System (Promega) according to the manufacturer’s recommendations. IV. RESULTS A. Extract Preparation High‐yield in vivo expression of biologically active recombinant protein is frequently achieved in cell lines derived from the fall army worm Sf or from the cabbage looper Trichoplusia ni (Vaughn, Goodwin, Tompkins, & McCawley, 1977). Recombinant baculoviruses containing the gene of interest are usually propagated in these insect‐derived cell lines.

1982). The preparation and analysis of protein cystals. New York: Wiley. McPherson, A. (1985). Crystallization of macromolecules: General principles. Methods in Enzymology, 114, 112–120. McPherson, A. (1989). Macromolecular crystals. Scientific American, 260(3), 62–69. McPherson, A. (1999). Crystallization of biological macromolecules. Cold Spring Harbor, NY: Cold Spring Harbor Press. , et al. (1986). An experiment regarding crystallization of soluble proteins in the presence of beta‐octyl glucoside.

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Current Topics in Membranes, Volume 63 by Larry DeLucas


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