By Fritz H. Bach, Robert A. Good
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Extra resources for Clinical immunobiology. Vol. 3
IgM K Λ E. I g D λ Μ Μ Li JÛJI F. I g C κ MM -. Fig. 1. Electrophoretic (top pattern in each panel) and selected immunoelectro phoretic serum patterns typical of (A) normal serum; ( B ) IgG κ myeloma; ( C ) IgM K macroglobulin; ( D ) IgA λ myeloma; ( E ) IgD λ myeloma; ( F ) IgE κ myeloma. In each figure only the abnormal arcs are indicated; the background normal bands are ignored. In the normal pattern only IgG, IgM, and IgA are shown for simplicity. ) An additional word of caution is warranted, since it is generally recog nized that the commonly used Albustix dip-stick test often fails to de tect Bence-Jones proteins.
The mean values, standard deviations, and ranges for the four subclasses are listed in Table III. For IgG4, only the mean and range are given. 2% IgG4. The distribution of the serum concentrations for IgGl, IgG2, and IgG3 were approximately symmetric. 9 mg/ml, was much wider than that for the other three subclasses. This has been found also in studies of other laboratories. Similar values were reported by a number of investigators. 2 100 45 SERUM CONCENTRATIONS OF IgG SUBCLASSES particularly of IgG3, listed in Table III, however, are somewhat lower than those found by others.
After completion of the electrophoretic separation, specific antisera are added to the wells parallel to the axis of electrophoresis and allowed to react with the individual pro tein components. For routine analysis, antisera to whole serum, γ, a, μ, κ, and λ chains are used and identify virtually homogeneous components. Because of the rarity of IgD and IgE myelomas, these antisera need not be used in the initial screening, but they should be employed in the rare event that an expected homogeneous arc is not noted with the six antisera used.
Clinical immunobiology. Vol. 3 by Fritz H. Bach, Robert A. Good