P. Michael Conn, Anthony R. Means (Eds.)'s Cellular Regulators Part B: Calcium and Lipids PDF

By P. Michael Conn, Anthony R. Means (Eds.)

ISBN-10: 0121820416

ISBN-13: 9780121820411

The seriously acclaimed laboratory typical, Methods in Enzymology, is among the such a lot hugely revered guides within the box of biochemistry. considering that 1955, every one quantity has been eagerly awaited, usually consulted, and praised by way of researchers and reviewers alike. The sequence includes a lot fabric nonetheless correct this day - really a necessary ebook for researchers in all fields of existence sciences

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Chem. 236, 54 (1961). 6t R. V. Tomlison and C. E. Ballou, J. Biol. Chem. 236, 1902 (1961). 62 R. F. Irvine, K. D. Brown, and M. J. Berridge, Biochem. J. 221, 269 (1984). 4 W Insll,4~5)P3 FIG. 3. Ins(1,4,5)P3-induced Ca 2+ release from the SR of skinned fibers. Redrawn with permission from Volpe e t al. 56 A segment of a chemically skinned fiber of the rabbit adductor is loaded with Ca 2+ (pCa 6A), rinsed in solution W, and then challenged with Ins(1,4,5)P3: 1 and 20/xM in the upper and lower trace, respectively.

Simon, in "Ion Selective Electrodes in Analytical Biochemistry" (H. ), Vol. 1, p. 211. Plenum, New York, 1978. ~4 N. Kamo, M. Muratsugu, R. Hongoh, and Y. Kobatake, J. Membr. Biol. 49, 105 (1979). 32 MEASUREMENT AND PERTURBATION OF CALCIUM [3] ing effect of the salt solution, For practical purposes Eq. (1) may therefore be rewritten as follows: Eta = Ec, + s log Cca (2) For an ideal Ca 2÷ electrode, Eq. 58 mV (at 25 °) (3) In practice s is about 95% of the theoretical value and electrodes with less than 90% theoretical slope should be discarded.

3 and 4, we used Ins(1,4,5)P3 prepared from erythrocyte ghosts as described by Downes and Michell. 58 +~ 30 0 Ins(1A,5)P3 o E C 20 10 0 itl 2I minI TC FIG. 4. Ins(1,4,5)P3-induced Ca 24 release from isolated TC. Redrawn with permission from Volpe e t a l : 6 Ca 2+ fluxes are followed with a Ca 2÷ electrode. LM Ins(1,4,5)P3 is added. Dotted line represents the control experiment. [1] CALCIUM RELEASE FROM SARCOPLASMIC RETICULUM 17 InsP3-1nduced Ca 2+ Release from the SR of Skinned Fibers Ca 2+ release from the SR is measured indirectly by following tension development.

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Cellular Regulators Part B: Calcium and Lipids by P. Michael Conn, Anthony R. Means (Eds.)


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