By Fernando González-Andrés, Euan James
This e-book covers the latest advances in the entire themes with which researchers and execs have to be generic with a view to receive a greater realizing of, and to higher take advantage of, helpful plant-microbe interactions. using microorganisms for agriculture and environmental purposes is gaining value world wide to enhance crop functionality, but in addition for different environmental purposes, comparable to bioremediation in chemically polluted soils. the hunt for an equilibrium among basic and utilized facets makes this booklet helpful for pros at a number of degrees within the worth chain of the “microbial biofertilizers”. demanding situations of comercializing biofertilizers contain potency of the goods and defense for human health and wellbeing and the surroundings, themes that experience paid valuable cognizance during this e-book. scholars, scientists and biofertilizers builders will locate up-to-date and complete information regarding different elements to be thought of to handle a winning advent of biofertilizers in sustainable agriculture and environmental actions.
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Additional info for Biological Nitrogen Fixation and Beneficial Plant-Microbe Interaction
Cold Spring Harb Perspect Biol 3:a001438 Srivastava R, Govil M (2007) Influence of biofertilizers on growth and flowering in gladiolus cv. American beauty. Acta Hortic 742:183–188 Storey EP, Boghozian R, Little JL, Lowman DW, Chakraborty R (2006) Characterization of ‘Schizokinen’; a dihydroxamate-type siderophore produced by Rhizobium leguminosarum IARI 917. Biometals, 19(6): 637–649 Vincent JM (1970) A manual for the practical study of root nodule bacteria. Blackwell Scientiﬁc Publications, Oxford Yanni YG, Rizk Y, El-Fattah Faiza KA et al (2001) The beneﬁcial plant growth-promoting association of Rhizobium leguminosarum bv.
05 M NaOH. Cell suspensions were held in a thermoblock for 4 min at 100 °C and 200 µl of sterile ultrapure water was ﬁnally added. 3 L. Celador-Lera et al. Random Ampliﬁed Polymorphic DNA (RAPD) Fingerprints The RAPD proﬁles were obtained as previously described (Rivas et al. 2002), using the M13 primer (5′ CAGGGTGGCGGTTCT 3′). 5 µl M13 primer (20 µM), and 2 µl DNA. PCR conditions were set as follows: preheating at 95 °C for 9 min; 35 cycles of denaturing at 95 °C for 1 min; annealing at 45 °C for 1 min and extension at 75 °C for 2 min, and a ﬁnal extension at 72 °C for 7 min.
All these mentioned legumes are worldwide used in human diet, but Pisum sativum (peas) is particularly appreciated by the high nutritional value of its seeds being widely cultivated in north Spain where more than 36,000 ha are cultivated in “Castilla y León” region. P. sativum establishes nitrogen-ﬁxing symbiosis with fast-growing rhizobia belonging to genus Rhizobium, but this symbiosis has been scarcely studied in most countries. Currently, the identiﬁcation of the P. sativum endosymbionts at species level is carried out by housekeeping gene analysis because several species nodulating this legume have identical 16S rRNA genes as occurs in the case of R.
Biological Nitrogen Fixation and Beneficial Plant-Microbe Interaction by Fernando González-Andrés, Euan James